Created at 1:12 p.m. Apr, 11, 2025
Author:
tia012
Type of change:
Updated content
Rationale for change
Clarity
this change is to clarify the steps taken to degrade or silence target mRNA that are outlined in the "extra space"
- dsRNA is transfected into human cells
- Dicer enzyme cleaves dsRNA into small short-interferingRNA(siRNA) or microRNA(miRNA)
- Small RNAs associate with RISC (RNA-induced silencing complex)
- RISC binds to target complementary mRNA
1. exact match: cleave mRNA -> degradation
2. partial match: inhibit ribosome and transcription factor binding -> inhibit translation
Source: AMBOSS - https://next.amboss.com/us/article/gp0FKS?q=%22DICER%22%20enzyme#Z66094bc0019892e00024efba7806592e
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