Rejected
at 11:20 p.m. Aug, 04, 2024 ]
by
Brian_BH
Author:
caffeinatednurse
Type of change:
New Tags
Rationale for change
SDS and reducing agents denature proteins. When analyzed by reducing SDS-PAGE, multimeric proteins will lose their quaternary structure and run as individual monomers.
Rejection reason
Bulk rejection
No changes in fields
Text
SDS-PAGE denatures proteins and separates them solely based on {{c1::mass}}.
Extra
- Proteins (negatively charged due to SDS) move to positive electrode.
- Proteins separate by size.
- Smaller proteins move faster—this allows you to estimate the protein’s molecular mass.
- Proteins separate by size.
- Smaller proteins move faster—this allows you to estimate the protein’s molecular mass.
Lecture Notes
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Missed Questions
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Pixorize
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Sketchy
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Additional Resources
One by one
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